The Lions missed the opportunity to take an advantage over Malatyaspor during the Istanbul match.
On Wednesday, Akhisarspor will play against Umraniyespor in the semifinals.
The second-leg matches will be played on April 24-25.
Three clubs from Turkish football's top-tier Super Toto Super Lig and a team from second-tier side Umraniyespor are into the semifinals.
In the last 16 round, Umraniyespor managed to eliminate Istanbul giants Fenerbahce, and in the quarterfinals, they eliminated Turkey's top-tier football league club Trabzonspor.
Galatasaray advanced to semifinals in the Turkish Cup after eliminating Turkish second-tier side Hatayspor 4-4 on aggregate.
The Lions are the most successful club in Turkish Cup history, amassing 17 titles as the winners.
The last Turkish Cup winners in 2018, Akhisar, eliminated Kasimpasa while Evkur Yeni Malatyaspor knocked out Goztepe in quarterfinal matches.
The final will be played in central Sivas province on May 16.
